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Inductively coupled plasma mass spectrometer (Agilent technologies 7700 series, USA) was used for quantitative analysis of the total ruthenium contents from different samples and cells

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All-atom molecular dynamics simulations reveal that AtSNX1 has a lower binding affinity to membranes compared to MmSNX1 The length of tubules generated by SNX1 proteins is influenced by several factors, particularly the membrane-binding affinity of SNX1, which is determined by its AH and directly impacts tubule stability and length 37