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doi:10.1038/ng.3696 Lyons JJ, Greiner G, Engel N, et al

After treating the sections at 37 C with binding buffer (Composition in mmol L 1 : HEPES 20, NaCl 150, MgCl 2 5, ethylene glycol-bis (-aminoethyl ether)- N , N , N ', N '-tetraacetic acid 1, and 0.1% BSA) for 60 min, we incubated the vagal branch with 15 nM 125 I-ghrelin or 15 nM 125 I-GLP-1 for 30 min

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