The proline substitutions at positions 25, 28 and 29 reduce fibrillation propensity relative to native amylin but do not abolish it, so avoid mechanical shear, avoid leaving concentrated solution standing at room temperature, and inspect for haze, filaments or opalescence before use
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The behaviour of the final solution also depends on the peptides purity, salt form, residual solvents, counterions, lyophilisation quality, moisture exposure, storage history, excipients, sequence length, hydrophobicity, and pH profile
Domingo JC, Cordobilla B, Ferrer R, Giralt M, Alegre-Martn J, Castro-Marrero J