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The samples were incubated at 25C for 5 min

10 mg Vial - Reconstitution Math BAC Water Added 1.0 mL Concentration 10 mg/mL 250 mcg 0.025 mL (2.5 u) 500 mcg 0.05 mL (5 u) 750 mcg 0.075 mL (7.5 u) 1 mg 0.10 mL (10 u) BAC Water Added 2.0 mL Concentration 5 mg/mL 250 mcg 0.05 mL (5 u) 500 mcg 0.10 mL (10 u) 750 mcg 0.15 mL (15 u) 1 mg 0.20 mL (20 u) BAC Water Added 3.0 mL Concentration 3.33 mg/mL 250 mcg 0.075 mL (7.5 u) 500 mcg 0.15 mL (15 u) 750 mcg 0.225 mL (22.5 u) 1 mg 0.30 mL (30 u) BAC Water Added 5.0 mL Concentration 2 mg/mL 250 mcg 0.125 mL (12.5 u) 500 mcg 0.25 mL (25 u) 750 mcg 0.375 mL (37.5 u) 1 mg 0.50 mL (50 u) 2 mL is the most common setup because it gives clean unit math at 500 mcg (10 units)

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