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Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

Methods Somatostatin secretion was measured from perfused mouse pancreases isolated from wild-type as well as diphtheria toxin-induced alpha cell knockdown, and global glucagon receptor knockout ( Gcgr / ) mice

2010;140(12):2302S-11S doi: jn.110.124958 [pii] 10.3945/jn.110.124958 [published Online First: 2010/10/15]

and excessive and prolonged oxidation arrest cell cycle triggering cell death (68, 72, 73)