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17 Indeed, pharmacologic responses to GIPRAs alone in patients with T2DM have been clinically disappointing with respect to improvements in glucose and cardiorenal outcomes

In particular with the CRISPRa approach, the transcriptional activators that are fused to the dCas9 enzyme will increase the length of the construct, such that a combination of sgRNA and dCas9-transcriptional activators will be too large to load onto a single AAV vector