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DG-75, BL-2, and Jurkat were cultured in RPMI 1640 medium (21875034), whereas HEK-293T were cultured in Dulbeccos modified Eagles medium (DMEM) (11965092), both from ThermoFisher Scientific (Waltham, MA, USA)

The use of the biological markers of alcohol drinking (GGT), antioxidant depletion (GSH) and hepatic damage (MDA), may be of help for the risk assessment in ALD