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[28] Tightly bound cofactors are, in general, regenerated during the same reaction cycle, while loosely bound cofactors can be regenerated in a subsequent reaction catalyzed by a different enzyme

Thus, in obese men, weight loss (with diet) improves semen quality, and ongoing GLP-1 treatment helps maintain those gains if lifestyle alone is insufficient to keep the weight off

Bromelain: A proteolytic enzyme and its clinical application

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