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Across the phase 3a PIONEER trials, less subjects with oral semaglutide (2.9%) had allergic reactions compared with the comparators (4.6%) (131)

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An equivalent amount of protein from each animal was mixed with 4 sample buffer with or without 2-mercaptoethanol, then separated by SDSpolyacrylamide gel electrophoresis, and transferred electrophoretically to a PVDF membrane (Merck Millipore)

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