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2009;1A:S437

Nb35 was purified by nickel affinity chromatography as previously described, 25 followed by size-exclusion chromatography using a HiLoad 16/600 Superdex 75 column (GE Healthcare)

Furthermore, the changes of plasma antioxidants (GR and GPX) levels showed an opposite pattern of release compared to liver enzymes during this acute phase of dengue infection, where the peak reduction of both antioxidants enzyme levels were observed on the day five of admission suggesting that elevation of AST and ALT could be associated with the oxidative stress induced by the dengue virus [5]