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Cells were separated from muscle tissue debris by differential centrifugation and filtration (nylon cell strainers) prior to a final centrifugation step at 1500 x g for 3 min, then suspended in DMEM containing 10% normal horse serum (HS), 1% antibiotic-antimycotic mixture, and 0.5% gentamicin (DMEM-10% HS), and plated on poly-L-lysine and fibronectin-coated plates

10.3390/nu9050455 87 Garca-CorderoJ.MartinezA.Blanco-ValverdeC.PinoA.Puertas-MartnV.San RomnR.et al (2023)

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If youre looking to get after your weight loss goals, CLA, L-Carnitine & Green Tea from Applied Nutrition is ideal (alongside a balanced diet and regular exercise)