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In the current HepG2 cell model, in which prolonged exposure is not feasible, the use of relatively high QI concentrations in a short timeframe allows for the identification of underlying mechanisms and pathways, as has been done previously in similar studies with hepatic toxicants (Tafazoli and OBrien, 2009

As seen in the figure above, for glutathione assay, concentration of total glutathione is determined by not treating the samples and the standard with 4-vinylpyridine

There is no established optimal protocol

Neuroendocrine control of sleep