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Surgical stress-induced gut microbiota dysbiosis in mice has been related to dysfunctions in multiple neurotransmitter systems, particularly the serotonin [5-hydroxytryptamine (5-HT)] and gamma-aminobutyric acid (GABA) systems (Lu et al., 2022)

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Stable covalent bond with minimal ligand leakage High binding capacity, very low nonspecific binding Scalable Easily adjusts for sample size and automation Reproducible results Investigating protein structure and function Preparing recombinant proteins for X-ray crystallography Ideal for study of protein interactions with protein or DNA Immunization to raise antibodies against a protein of interest Effective screening protein expression even with crude cell lysates Microscale purification of GST-tagged proteins Glutathione-S-transferase (GST) is a highly soluble and stable 26 kDa enzyme that catalyzes the protective mechanisms of Glutathione (GSH)