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(2007, Growth Hormone & IGF Research ) confirmed that following 300 eccentric lengthening contractions of the knee extensors in healthy young men, MGF mRNA expression peaked at 24 hours post-exercise while IGF-1Ea mRNA rose later at 72 hours providing direct in vivo human evidence of the sequential IGF-1 splice variant programme and establishing the temporal window for MGF-mediated satellite cell activation in physiological muscle damage

Expression can fail for various reasons

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If vasomotor symptoms are prominent: Consider an SSRI/SNRI (especially if mood is also an issue), gabapentin (especially if pain is also an issue), or fezolinetant (especially if other options havent worked)