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The brains from the rats allocated for the histological study were preserved in 10% formalin overnight at 4 C to ensure proper fixation of the brain tissue, which was then processed into paraffin blocks and stored at room temperature until the examination of the hippocampal tissues

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Treatment should only be continued if there is a beneficial metabolic response (reduction of at least 11 mmol/mol [1%] in HbA1c and weight loss of at least 3% of initial body weight at 6 months)