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this effect will counteract the excessive ROS released by MTX, preserving the level of both enzymatic and non-enzymatic antioxidants.[] Furthermore, pomegranate causes Nrf2 translocation from cytosol to the nucleus, which may cause cytoprotective events and increase hepatic GSH and SOD levels as part of this event.[] As a result of the positive pomegranate effect on GSH and SOD and enhancement of antioxidant capability in the liver tissue, pomegranate will ameliorate the oxidative stress caused by MTX and a reduction of ROS induce lipid peroxidation, which was reflected by the significantly lower level of MDA in the liver tissue compared to the control group

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