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Semi-automated freeze-substitution was performed using an AFS2 device (Leica) equipped with customized aluminum sapphire disc revolvers and samples were subsequently embedded in epoxy resin according to a previously published protocol 73

Lipedema World Alliance Delphi Consensus-Based Position Paper on the Definition and Management of Lipedema: Results from the 2023 Lipedema World Congress in Potsdam

doi: 10.1074/mcp.M110.001560

Primary PK parameters included area under the plasma concentrationtime curve (AUC) from time zero to the last quantifiable concentration (AUC 0-last ) and extrapolated to infinity (AUC 0- ), and maximum observed concentration (C max )