Cell transfection Cells were transfected with pcDNA 3.1 overexpression vectors (pcDNA 3.1-YTHDF1 and pcDNA 3.1-SLC7A5, with the empty pcDNA 3.1 vector as a negative control) and siRNAs (Invitrogen) targeting YTHDF1 and SLC7A5 (si-YTHDF1, si-SLC7A5, with non-targeting siRNA (si-NC) as a negative control)
This is the dose where "forgetting to eat" becomes common, where portion sizes shrink noticeably, and where the relationship with food begins its most visible shift
Russo SJ, Bolanos CA, Theobald DE, DeCarolis NA, Renthal W, Kumar A, Winstanley CA, Renthal NE et al (2007) IRS2-Akt pathway in midbrain dopamine neurons regulates behavioral and cellular responses to opiates
42, 66, 159, 978, 984 Sandri, M