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The explanation for this discrepancy can be found in recent papers showing that some cancer cell linesincluding HCT116are not affected by L-BSO or GSH synthesis inactivation because they can compensate GSH depletion by the thioredoxin (TXN) pathway 25 , and by maintaining protein homeostasis through deubiquitinating enzymes (DUB) 32

Pregnancy is NOT a contraindication

Avoid GSH Depleters- Alcohol, smoking, and other GSH depletors reduce glutathione level in the body, and thus it is avoidable if you want to take its maximum absorption

Importantly, the gpoA , ahpD , and tpxD mutants showed a similar H 2 O 2 susceptibility, representing a 12-fold decrease compared to the wild-type ( wt ) strain (Fig 3B, Fig S2)