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The binding characteristics of F1 and F2 peptides To determine the binding characteristics of selected peptides (F1 and F2), an analysis of the interaction of synthesized peptides with glutathione Sepharose 4B beads was performed using the Scatchard method

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Then the absorbance was recorded at 593 nm via a power wave 340 ELISA Reader (Bio-Tek Instruments, Inc., Winooski, VT, USA)