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CRISPR-mediated knockout The sgRNA GPX4 lentiCRISPR v2 plasmid was a gift from Masafumi Takahashi (Jichi Medical University) 22 , and the sgRNA HMGCS2 lentiCRISPR v2 plasmid was constructed for us by the Duke Functional Genomics Core Facility (sgRNA sequence: sense 5 GATACTTGGCCAAAGGACGT 3)

The upregulated gene clusters are enriched in extracellular matrix components (collagens I and III, decorin, elastin), antioxidant defense systems (superoxide dismutase, glutathione-related enzymes), DNA repair pathways, and anti-inflammatory mediators including IL-10 and TGF-beta family members

Bathrooms are common locations, as these tests require urine

Sun exposure can lead to hyperpigmentation, irritation, or even reverse the progress youve made