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Subsequently, ChIP assays were performed using anti-p-CREB (cell signaling technology), anti-CBP (cell signaling technology), anti-H3K4me3 (cell signaling technology), or anti-H2AK119ub antibodies (Abcam, United Kingdom) and a negative control comprising normal rabbit IgG (Santa Cruz Biotechnology, Inc., United States)

Ipamorelin is a man-made pentapeptide, also known as NNC 26-0161, that is believed to associate with a specific receptor in the pituitary gland cells, termed the growth hormone secretagogue receptor (GHS-R1a)