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This creates a pro-ferroptotic microenvironment for osteoblasts and osteocytes, contributing to abnormal bone remodeling (56)

Cell culture Bone marrow-derived macrophages (BMDMs) were harvested from the femur and tibia of C57BL/6J mice and cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% fetal bovine serum (FBS, Gibco, Carlsbad, CA, USA) and 10% L929 cell-conditioned medium, and maintained in a humidified incubator with 5% CO 2 at 37 C for 7 days [36]

At a low dose (0.5 M), OTA exposes the cells to mitochondrial transmembrane potential loss and apoptosis (97)

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