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After incubation at room temperature for 20 min, the luminescence was measured with a GloMax Multi Detection System luminometer (Agilent, Santa Clara, United States)

Santofimia-Castao P, Garcia-Sanchez L, Ruy DC, Sanchez-Correa B, Fernandez-Bermejo M, Tarazona R, Salido GM, Gonzalez A (2015b) Melatonin induces calcium mobilization and influences cell proliferation independently of MT1/MT2 receptor activation in rat pancreatic stellate cells

The vial size determines how much bacteriostatic water you add, which determines the concentration of your final solution, which determines how many units you draw for each dose

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