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An aliquot of 5 l of this reaction was diluted to 180 l with reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA, 1 mM DTT) to attain final concentrations of 5 g ml 1 GSTO1 and 0.25 IC 50 of inhibitor
FAD, Flavin adenine dinucleotide
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Sterile technique, sterile supplies, and correct reconstitution matter see the dosage calculator for the math and our storage guide for handling
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