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Cell culture HEK293-GLP-1R-GFP cells (generated in-house using HEK293 cell line purchased from ATCC) were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum, 1 mg ml 1 geneticin and 100 g ml 1 hygromycin

doi: 10.5114/ADA.2017.71110 168 Dalle-DonneIAldiniGCariniMColomboRRossiRMilzaniA

These side effects are often most pronounced during the initial weeks of treatment or after dose increases

A saturation binding isotherm for exendin(9-39)-FITC was performed during each experiment, and the K i values for competing agonists were determined using the Fit K i preset of Prism 8.0