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Cultures were maintained in a humidified atmosphere of 5% CO 2 at 37C for 24 h and then incubated for the next 24-h period in DMEM-10% HS additionally containing recombinant HGF that was treated with peroxynitrite with or without trisulfide pre-treatment (see Figure 1d,f)

Multivariate analysis demonstrated that higher education and diagnosed cardiovascular disease were associated with achievement of therapeutic goals, whereas smoking was associated with worse control of arterial blood pressure and LDL-C concentration [300] (Section 13)

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This is the same logic behind loading protocols in many peptide therapies