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The restriction sites were introduced by PCR using the primer pairs 5-TGGATCCGCTCAAGAGTTTGTGAACTG-3 and 5-CTAAGCTTTTACTTGTACAGCTCGTCC-3 for GRX1-roGFP2iL and 5-AAGGATCCGTGAGCAAGGGCGAGGAGC-3 and 5-CTAAGCTTTTACTTGTACAGCTCGTCC-3 for roGFP2-iL

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To interrogate the mechanisms underlying NRF2-directed regulation of this core gene set, we compared mean promoter (1 kb restricted) ChIP-seq signal in A549 for NRF2, p300, as well as the histone marks H3K4me3 and H3K27ac, which are enriched at promoters and enhancers, respectively

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