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The reactions were incubated at 25 C for 20 min to allow telomerase to synthesise the TRAP ladders, then the qPCR was carried out at 95 C for 10 min and 35 cycles of 95 for 30 s, and 60 for 90 s

coli , a similar type of approach as ours gave approximately three-fold enhancement of production of free fatty acid and tyrosine [20], versus an ~ 18% increase in GSH production in the present study with yeast

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