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In addition, they showed that the 5-hydroxytryptamine 2A (5-HT 2A ) receptor is crucial for GLP-1RA-induced weight loss after exendin-4 ICV injection, such as peripheral injection of liraglutide in rats

Dosing tiers: Morning dosing maximizes synergy with daytime physical activity

An intra-colonic injection of 180 mmol l 1 propionate (a total dose of ~0.45 mmol) vs saline control (matched for sodium content and pH) in anaesthetised rats resulted in a significant rise in circulating plasma PYY and GLP-1 levels (two-way ANOVA, effect of treatment P =0.024 and P =0.023, respectively)

It is for understanding the options, not a protocol to follow on your own