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50 mM iodoacetic acid was added to prevent the formation of disulfide bonds of reduced cysteine residues, and samples were stored at 80C

The VKDB in these cases is usually mild

However, individuals with darker skin tones often require 6-12 months of consistent therapy to achieve pronounced changes in pigmentation

This bacteriostatic rather than bactericidal preservative action maintains solution sterility between multiple vial access events and the defining practical advantage of Bacteriostatic Water over unpreserved Sterile Water for Injection, which must be used immediately upon vial entry or discarded to prevent microbial contamination