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RNA was reverse transcribed using High-capacity RNA-cDNA reverse transcription Kit on a Mini Amp Plus Thermal Cycler (Thermo Fisher, Waltham, MA), and qRT-PCR was performed using TaqMan Fast Advanced master mix on a CFX96 Real-Time System (Bio-Rad, Hercules, CA)

Zinc thymulin is a peptide-mineral complex that combines zinc with thymulin, a naturally occurring peptide that has been studied in skin and scalp-related research contexts.In cosmetic formulations, zinc thymulin is commonly used to support scalp balance and hair appearance

The time until extreme bradycardia and asystole was assessed

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