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In 1966 (the manuscript was actually received by the journal on 10 April 1965), Bruno Jirgensons reported a comprehensive analysis of multiple proteins by optical rotatory dispersion (ORD) to classify them according to the conformation of their polypeptide chains (Jirgensons, 1966)

1419) (Diagen, Ljubljana, Slovenia) dissolved in 0.9% NaCl was used in all experiments [1,2,3,4,5,6,7,8,9,10,11]