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GPX5 is described in the epididymis as a secreted protein that contains cysteine (Cys) in the active center instead of Sec, GPX5 is the first discovered Cys-based superfamily member, also known as epididymal GPX (EGPX), which has now been shown to play a role in protecting mouse sperm from hydrogen peroxide toxicity (Zhang et al., 2008
This, in turn, can compromise the structural and functional integrity of the cells nucleic acids, proteins and lipids [4]
Advances in metabolomics and molecular biology are expected to further uncover the complex interplay between metabolism and fibrosis, offering new opportunities for biomarker discovery, early diagnosis, and the development of targeted, personalized treatments for pulmonary fibrosis
Secondary antibodies (AlexaFluor 594 anti-mouse 1:500 (Invitrogen, A11005) and AlexaFluor 488 anti-rabbit 1:500 (Invitrogen, A11034)) were added to blocking buffer and incubated at room temperature for 1 h
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