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The relative expression levels of the genes were analyzed using the 2 -Ct method, where Ct = (average Ct value of the target gene in the experimental group - average Ct value of the reference gene in the experimental group) - (average Ct value of the target gene in the control group - average Ct value of the reference gene in the control group) 59,102

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Induced pluripotent stem cell-derived MSCs (iPSC-MSCs) activate CD4+ and CD8 + T cells, upregulate and activate Treg cells, and balance Th1/Th2 cytokine levels without any additional stimulation [147]