2 2.8.3 Widely targeted metabolomics analysis Frozen tissue specimens were homogenized and extracted using ice-cold methanol:water (7:3, v/v) supplemented with isotope-labeled internal standards,followed by multi-platform LC-MS/MS analysis
Mix protocols can reveal combined effects where the combined response exceeds the sum of personal effects, antagonistic interactions where one peptide tunes anothers effects, or paired mechanisms where different peptides affect distinct but related processes
The in vitro analyses of the IgE recognition were performed by RAST inhibition assay using sera with RAST values to AXO-poly-L-lysine (AXO-PLL) higher than 7%
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