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Meta-analytic synthesis of randomized controlled trials has also complemented observational evidence

Prior to initiation of treatments, mice were randomized and distributed into groups allowing similar mean values of body weight and fat mass

117 Interestingly, glucagon also exerts insulinotropic effects on islet beta-cells, and this is likely to allow for tissues to uptake glucose and fat once glucagon has induced their catabolism from nutrient storage (as tissues such as skeletal muscle rely on insulin for nutrient uptake from circulation), or the insulinotropic effects of glucagon could simply serve as a feedback inhibition mechanism