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In the electron transport chain (ETC), FMN and FAD function as cofactors that shuttle electrons, acting especially in I and II complexes, which contain flavoproteins reductases and electron transferring flavoproteins, thereby facilitating the production of adenosine triphosphate (ATP), the primary energy source of cells, being particularly important in the nervous system, due to its high energy demands (3, 43, 45)

If possible, reduce the dilution factor of the pretreatment or increase the sample volume to increase the concentration of the sample above the measuring range

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