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Due to neurons having a limited capacity for GSH synthesis, they must rely on astrocytes to supply precursors such as cysteine ( 3.3 Parkinsons disease PD is a neurodegenerative disorder that typically presents later in life with generalized bradykinesia and another symptom of resting tremor or rigidity

Tissue homogenates were transferred into calibrated Oxygraph-2k (O2k, Oroboros Instruments), and DatLab software (v.7.4, Oroboros Instruments) was used to record real-time oxygen concentration (M), as well as oxygen flux per tissue mass (pmol O 2 s 1 mg 1 )

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The pellet was resuspended in 0.1 M K 2 P 2 O 7 , 1 mmol/L EDTA (pH 7.4) and centrifuged again for 65 min at 105,000 g to give the final microsomal fraction