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18 O incorporation into cyanidin (8) Flavan-3,3,4-triol ( 4 ) was first formed in a reaction with 100 mM MOPSO, pH 6.2, 20 mM sodium ascorbate, 1 mM -ketoglutaric acid, 0.4 mM ammonium iron(II) sulfate, 500 M 3,4- cis -leucocyanidin ( 6 ) and 2.5 M At LDOX, which was incubated at 30 C for 15 min at 1,200 rpm using a 3 mm shaking diameter

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Pre-mixed vials lock the ratio they cannot be used during the staged titration described above without overdosing one component or underdosing the other