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All animal procedures and protocols were approved by the Animal Care and Use Committee of the University of Science and Technology of China

Analytical Verification Every batch undergoes Mile High Compounds comprehensive analytical protocol: High-Performance Liquid Chromatography (HPLC) for purity quantification of each component Mass Spectrometry (MS) for molecular weight and identity confirmation of both peptides 14-day sterility testing Endotoxin analysis 4-vial batch conformity verification statistically robust consistency testing across each production batch, exceeding the industry-standard single-vial sampling approach Heavy metal screening for lead, arsenic, cadmium, mercury, and additional trace metals Container and seal integrity verification Batch-specific Certificates of Analysis (COAs) accompany every order, documenting full testing results for both component peptides

These include vitamins B2, B3, B5, selenium, zinc, and vitamin C

The receiver operating characteristic curve was used to define the cut-off point