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Determination of plasma IL-1 and TNFalpha levels A venous blood sample was withdrawn from the vena cava into EDTA-containing vials in order to determine the plasma level of interleukin-1 beta (IL-1) and tumor necrosis factor alpha (TNFalpha) by ELISA technique (BioSource International, Camarillo, CA, USA)

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Laparoscopy: A doctor might also perform a laparoscopy