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In fourth embodiment, the present invention is related to a process for the preparation of semaglutide, comprising the steps of: a) culturing a host cell comprising a nucleotide sequence encoding of Formula (II) or Formula (III) under suitable conditions for expression, Insoluble tag Fusion tag Semapeptide (Nucleotide Sequence) Formula (II) Affinity Tag Insoluble tag Fusion tag Semapeptide (Nucleotide Sequence) Formula (III) wherein, insoluble tag is nucleotide sequence of Alanine -Valine