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PMID 11443939

Red dotted lines indicate hydrogen bonds

CETSA in intact cells (In-cell CETSA) Jurkat cells (adjusted to 2 10 6 cells/ml, 5 ml/flask) were seeded in two T25 tissue culture flasks and treated with 10 M compound or vehicle (DMSO), with 0.1% DMSO adjusted, for 15 min at 37 C

Cysteine anabolism and its interplay with other metabolic pathways in cancer De novo cysteine synthesis The de novo synthesis of cysteine occurs through the trans-sulphuration pathway of the methionine cycle, which also involves serine (Fig