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Cell Culture Immortalized mouse hippocampal cell line HT22 was cultivated in high-glucose DMEM (C11995500BT, Gibco, United States) containing 10% FBS (10270-106, Gibco, United States), 100 U/ml penicillin and 100 g/ml streptomycin that maintained in a 5% CO 2 incubator at 37C

Glycemic indexes (A1C, fasting blood glucose) and kidney function should be monitored during initiation of therapy and routinely throughout the treatment plan

To determine the optimal concentration of Ex-4, we treated the steatotic cells with increasing concentrations of Ex-4 from 0 to 1 mM and with different incubation periods (3, 6, 12, and 24 h)