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Imaging was performed with a BX61WI (Olympus) microscope equipped with a Chameleon Ultra (COHERENT) two photon laser and a 25 water lens (Olympus, UIS2, N.A.1.05)

7.2.6 Economic analysis of health interventions In resource-constrained settings, comprehensive cost-effectiveness analyses are needed to guide the integration of novel therapies into clinical guidelines and public health policies

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First-strand cDNA synthesis was performed with 1 g of total RNA in 20 l reactions using an iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA)