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Besides its cofactor role, NAD + is also a substrate for NAD + -consuming enzymes such as sirtuins (regulators of gene expression and longevity pathways) and PARPs, which mediate DNA repair and cellular stress responses

Low testosterone affects an estimated 4 to 5 million American men, though many cases go undiagnosed (Mulligan et al., 2006)

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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development