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Preservative-free formulations, often requested for sensitive individuals, almost always require refrigeration

The method of embodiment75, wherein the composition comprises between about 0.01% to about 20% by weight of the cholesterol, about 0.01% to about 15% by weight of the cholesterol, about 0.01% to about 10% by weight of the cholesterol, about 0.01% to about 7% by weight of the cholesterol, about 0.01% to about 5% by weight of the cholesterol, about 0.01% to about 3% by weight of the cholesterol, about 0.01% to about 2% by weight of the cholesterol, or about 0.01% to about 1% by weight of the cholesterol

PMC 8395423

Injecting slowly over 5-10 seconds largely prevents this