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Stabilized values of this index suggest a good functional state of the kidneys of the volunteers included in the presented studies

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Proteins were extracted by using RIPA buffer supplemented with phosphatase and protease inhibitors, quantified by using Bradford method (23236, ThermoFisher)

And PE is able to establish hydrogen bonds with a variety of amino acid residues, which in turn inhibits the localization of negative amino acids on the cytoplasmic side, and is an important determinant of the topology of transmembrane structural domains of membrane proteins (Shi et al., 2024)