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Once internalized by the cell through the folate receptor (RFC1), MTX undergoes sequential glutamylation by folyl-polyglutamate synthase (FPGS), thus becoming MTX polyglutamates (MTX-PG 2-6 )
Molecular modelling analyses based on molecular mechanics, semi-empirical and DFT (at B3LYP/6-31G* level) calculations show that there are both electron-rich and electron-deficient regions on the surfaces of NAP and its metabolites so that they may be subject to both electrophilic and nucleophilic attacks
We also describe challenges and limitations that we envision we will encounter when delivering this course, namely in examination requirements, and transversal individual and group learning
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![Structure of [ 3 H]liraglutide (top) and the GLP-1 receptor agonists](https://www.researchgate.net/profile/Inga-Bjornsdottir-2/publication/45650716/figure/fig2/AS:667822585806848@1536232720030/Structure-of-3-Hliraglutide-top-and-the-GLP-1-receptor-agonists-liraglutide-and.png)
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